Mouse cytomegalovirus is infectious for rats and alters lymphocyte subsets and spleen cell proliferation
Identifieur interne : 001611 ( Main/Exploration ); précédent : 001610; suivant : 001612Mouse cytomegalovirus is infectious for rats and alters lymphocyte subsets and spleen cell proliferation
Auteurs : C. B. Smith [États-Unis] ; L. S. Wei [États-Unis] ; Marie Griffiths [États-Unis]Source :
- Archives of Virology [ 0304-8608 ] ; 1986-09-01.
English descriptors
- Teeft :
- Adult rats, Background spleen cell cultures, Cell cultures, Cell numbers, Cell ratios, Cell suspensions, Control animals, Control rats, Control values, Cytomegalovirus, Immune response, Incubation period, Infection, Inoculation, Laboratory rats, Larger inoculum, Lymphocyte, Lymphocyte reactivity, Lymphocyte subsets, Marie griffiths, Mcmv, Mcmv antigen, Mcmv control, Mcmv infection, Mitogen, Monoclonal antibody, Mouse cytomegalovirus, Neutralizing antibodies, Normal mouse, Peripheral blood, Rat, Salivary gland tissue, Salt lake city, Separate experiments, Several strains, Smith strain, Spleen, Spleen cell, Spleen cell proliferation, Spleen cell reactivity, Spleen cells, Subset, Unstimulated cultures.
Abstract
Summary: The Smith strain of mouse cytomegalovirus (MCMV) was infectious for infant and mature DA strain laboratory rats as judged by development of neutralizing antibodies and specific spleen cell proliferation on stimulation with MCMV antigen. An i. p. inoculum of 106 PFU of MCMV was fatal for more than two-thirds of infant mice (1–7 days of age), and disseminated viral infection was documented by isolation of virus from body organs. In contrast, weanling and adult rats did not become ill as a result of infection with a larger inoculum of 107 PFU. However, these older MCMV infected rats did show transient reversals of T helper/suppressor cell ratios and alterations of immune cell function as detected byin vitro spleen cell proliferation assays. Seven days after MCMV infection, there was a generalized increase in3H-thymidine incorporation by spleen cells in both resting (unstimulated) cultures and cultures exposed to mitogens (Con A, PHA, LPS) and to MCMV antigen. At 14 days, the spleen cell proliferation in the unstimulated cultures returned to normal but was depressed compared to controls in response to Con A. These observations show that laboratory rats are susceptible to MCMV infection and that assymptomatic infection may occur and cause transient alterations in lymphocyte subsets and in their reactivity to mitogens.
Url:
DOI: 10.1007/BF01317379
Affiliations:
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Wei</name></author><author><name sortKey="Griffiths, Marie" sort="Griffiths, Marie" uniqKey="Griffiths M" first="Marie" last="Griffiths">Marie Griffiths</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:A91D3B988E1648721364B167FD8969123EA190E1</idno><date when="1986" year="1986">1986</date><idno type="doi">10.1007/BF01317379</idno><idno type="url">https://api.istex.fr/ark:/67375/1BB-KNZVTNHK-G/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000577</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000577</idno><idno type="wicri:Area/Istex/Curation">000557</idno><idno type="wicri:Area/Istex/Checkpoint">000747</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000747</idno><idno type="wicri:doubleKey">0304-8608:1986:Smith C:mouse:cytomegalovirus:is</idno><idno type="wicri:Area/Main/Merge">001635</idno><idno type="wicri:Area/Main/Curation">001611</idno><idno type="wicri:Area/Main/Exploration">001611</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Mouse cytomegalovirus is infectious for rats and alters lymphocyte subsets and spleen cell proliferation</title><author><name sortKey="Smith, C B" sort="Smith, C B" uniqKey="Smith C" first="C. B." last="Smith">C. B. Smith</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Veterans Administration Medical Center and Department of Medicine, University of Utah School of Medicine, Salt Lake City, Utah</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author><author><name sortKey="Wei, L S" sort="Wei, L S" uniqKey="Wei L" first="L. S." last="Wei">L. S. Wei</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Veterans Administration Medical Center and Department of Medicine, University of Utah School of Medicine, Salt Lake City, Utah</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author><author><name sortKey="Griffiths, Marie" sort="Griffiths, Marie" uniqKey="Griffiths M" first="Marie" last="Griffiths">Marie Griffiths</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Veterans Administration Medical Center and Department of Medicine, University of Utah School of Medicine, Salt Lake City, Utah</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Virology</title><title level="j" type="abbrev">Archives of Virology</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Vienna</pubPlace><date type="published" when="1986-09-01">1986-09-01</date><biblScope unit="volume">90</biblScope><biblScope unit="issue">3-4</biblScope><biblScope unit="page" from="313">313</biblScope><biblScope unit="page" to="323">323</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Adult rats</term><term>Background spleen cell cultures</term><term>Cell cultures</term><term>Cell numbers</term><term>Cell ratios</term><term>Cell suspensions</term><term>Control animals</term><term>Control rats</term><term>Control values</term><term>Cytomegalovirus</term><term>Immune response</term><term>Incubation period</term><term>Infection</term><term>Inoculation</term><term>Laboratory rats</term><term>Larger inoculum</term><term>Lymphocyte</term><term>Lymphocyte reactivity</term><term>Lymphocyte subsets</term><term>Marie griffiths</term><term>Mcmv</term><term>Mcmv antigen</term><term>Mcmv control</term><term>Mcmv infection</term><term>Mitogen</term><term>Monoclonal antibody</term><term>Mouse cytomegalovirus</term><term>Neutralizing antibodies</term><term>Normal mouse</term><term>Peripheral blood</term><term>Rat</term><term>Salivary gland tissue</term><term>Salt lake city</term><term>Separate experiments</term><term>Several strains</term><term>Smith strain</term><term>Spleen</term><term>Spleen cell</term><term>Spleen cell proliferation</term><term>Spleen cell reactivity</term><term>Spleen cells</term><term>Subset</term><term>Unstimulated cultures</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: The Smith strain of mouse cytomegalovirus (MCMV) was infectious for infant and mature DA strain laboratory rats as judged by development of neutralizing antibodies and specific spleen cell proliferation on stimulation with MCMV antigen. An i. p. inoculum of 106 PFU of MCMV was fatal for more than two-thirds of infant mice (1–7 days of age), and disseminated viral infection was documented by isolation of virus from body organs. In contrast, weanling and adult rats did not become ill as a result of infection with a larger inoculum of 107 PFU. However, these older MCMV infected rats did show transient reversals of T helper/suppressor cell ratios and alterations of immune cell function as detected byin vitro spleen cell proliferation assays. Seven days after MCMV infection, there was a generalized increase in3H-thymidine incorporation by spleen cells in both resting (unstimulated) cultures and cultures exposed to mitogens (Con A, PHA, LPS) and to MCMV antigen. At 14 days, the spleen cell proliferation in the unstimulated cultures returned to normal but was depressed compared to controls in response to Con A. These observations show that laboratory rats are susceptible to MCMV infection and that assymptomatic infection may occur and cause transient alterations in lymphocyte subsets and in their reactivity to mitogens.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Utah</li></region></list><tree><country name="États-Unis"><region name="Utah"><name sortKey="Smith, C B" sort="Smith, C B" uniqKey="Smith C" first="C. B." last="Smith">C. B. Smith</name></region><name sortKey="Griffiths, Marie" sort="Griffiths, Marie" uniqKey="Griffiths M" first="Marie" last="Griffiths">Marie Griffiths</name><name sortKey="Wei, L S" sort="Wei, L S" uniqKey="Wei L" first="L. S." last="Wei">L. S. Wei</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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